Device
Part:BBa_K317027
Designed by: Yuta Sakai Group: iGEM10_Tokyo-NoKoGen (2010-10-19)
OmpR positive promoter(BBa_R0082)-RBS-Antigen 43-Double terminator
Antigen 43 involves in auto-aggregation of the cell. By expressing Antigen 43, the cell will aggregate when the culture solution is stood. Antigen 43 is derived from E. coli genome. The wildtype of Antigen 43 contains 6 PstI sites but this part does not contain any of them. So, this parts can be used as a normal BioBrick which is able to ligate 2 parts by 3A assembly.
In this device, the expression of Antigen 43 regulate by OmpR positive promoter. This promoter can be activated by EnvZ.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 843
Illegal NgoMIV site found at 2067
Illegal NgoMIV site found at 2577
Illegal NgoMIV site found at 2598
Illegal AgeI site found at 1593
Illegal AgeI site found at 2337
Illegal AgeI site found at 2751
Illegal AgeI site found at 2993 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |